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MBF Bioscience unbiased stereology
Diminished efficacy with shorter and later treatment. Five weeks of treatment once daily with 50 mg/kg LM11A-31 (n = 5) beginning at 17 months of age partially prevented and/or reversed the decrease in cholinergic neuron area in the MS and VDB seen in the vehicle treated mice (n = 4). Cholinergic neurons were identified by ChAT immunoreactivity and an unbiased estimate of area was obtained using <t>Microbrightfield</t> <t>(MBF)</t> software. A significant decrease in cell area measurements was seen in all groups (t-test, (n = 8) vs 18 months (n = 4), p values = 0.018–0.001). Inhibition and/or reversal of loss of cell size after five weeks of treatment was partial and reached significance only in the composite data for MS + VDB (t-test, treated vs vehicle, p = 0.032). Values are expressed as mean + sem.
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MBF Bioscience stereology workstation
Diminished efficacy with shorter and later treatment. Five weeks of treatment once daily with 50 mg/kg LM11A-31 (n = 5) beginning at 17 months of age partially prevented and/or reversed the decrease in cholinergic neuron area in the MS and VDB seen in the vehicle treated mice (n = 4). Cholinergic neurons were identified by ChAT immunoreactivity and an unbiased estimate of area was obtained using <t>Microbrightfield</t> <t>(MBF)</t> software. A significant decrease in cell area measurements was seen in all groups (t-test, (n = 8) vs 18 months (n = 4), p values = 0.018–0.001). Inhibition and/or reversal of loss of cell size after five weeks of treatment was partial and reached significance only in the composite data for MS + VDB (t-test, treated vs vehicle, p = 0.032). Values are expressed as mean + sem.
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MBF Bioscience micro brightfield mbf camera
Diminished efficacy with shorter and later treatment. Five weeks of treatment once daily with 50 mg/kg LM11A-31 (n = 5) beginning at 17 months of age partially prevented and/or reversed the decrease in cholinergic neuron area in the MS and VDB seen in the vehicle treated mice (n = 4). Cholinergic neurons were identified by ChAT immunoreactivity and an unbiased estimate of area was obtained using <t>Microbrightfield</t> <t>(MBF)</t> software. A significant decrease in cell area measurements was seen in all groups (t-test, (n = 8) vs 18 months (n = 4), p values = 0.018–0.001). Inhibition and/or reversal of loss of cell size after five weeks of treatment was partial and reached significance only in the composite data for MS + VDB (t-test, treated vs vehicle, p = 0.032). Values are expressed as mean + sem.
Micro Brightfield Mbf Camera, supplied by MBF Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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MBF Bioscience 2d probe ‘mbf fractionator’
Diminished efficacy with shorter and later treatment. Five weeks of treatment once daily with 50 mg/kg LM11A-31 (n = 5) beginning at 17 months of age partially prevented and/or reversed the decrease in cholinergic neuron area in the MS and VDB seen in the vehicle treated mice (n = 4). Cholinergic neurons were identified by ChAT immunoreactivity and an unbiased estimate of area was obtained using <t>Microbrightfield</t> <t>(MBF)</t> software. A significant decrease in cell area measurements was seen in all groups (t-test, (n = 8) vs 18 months (n = 4), p values = 0.018–0.001). Inhibition and/or reversal of loss of cell size after five weeks of treatment was partial and reached significance only in the composite data for MS + VDB (t-test, treated vs vehicle, p = 0.032). Values are expressed as mean + sem.
2d Probe ‘Mbf Fractionator’, supplied by MBF Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MBF Bioscience optical fractionator probe from mbf’s stereo investigator software version 2017.03
Diminished efficacy with shorter and later treatment. Five weeks of treatment once daily with 50 mg/kg LM11A-31 (n = 5) beginning at 17 months of age partially prevented and/or reversed the decrease in cholinergic neuron area in the MS and VDB seen in the vehicle treated mice (n = 4). Cholinergic neurons were identified by ChAT immunoreactivity and an unbiased estimate of area was obtained using <t>Microbrightfield</t> <t>(MBF)</t> software. A significant decrease in cell area measurements was seen in all groups (t-test, (n = 8) vs 18 months (n = 4), p values = 0.018–0.001). Inhibition and/or reversal of loss of cell size after five weeks of treatment was partial and reached significance only in the composite data for MS + VDB (t-test, treated vs vehicle, p = 0.032). Values are expressed as mean + sem.
Optical Fractionator Probe From Mbf’s Stereo Investigator Software Version 2017.03, supplied by MBF Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/builder+for+java+mbfj/pmc10177146-76-15-21?v=MBF+Bioscience
Average 90 stars, based on 1 article reviews
optical fractionator probe from mbf’s stereo investigator software version 2017.03 - by Bioz Stars, 2026-06
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90
MBF Bioscience microbrightfield mbf camera
Diminished efficacy with shorter and later treatment. Five weeks of treatment once daily with 50 mg/kg LM11A-31 (n = 5) beginning at 17 months of age partially prevented and/or reversed the decrease in cholinergic neuron area in the MS and VDB seen in the vehicle treated mice (n = 4). Cholinergic neurons were identified by ChAT immunoreactivity and an unbiased estimate of area was obtained using <t>Microbrightfield</t> <t>(MBF)</t> software. A significant decrease in cell area measurements was seen in all groups (t-test, (n = 8) vs 18 months (n = 4), p values = 0.018–0.001). Inhibition and/or reversal of loss of cell size after five weeks of treatment was partial and reached significance only in the composite data for MS + VDB (t-test, treated vs vehicle, p = 0.032). Values are expressed as mean + sem.
Microbrightfield Mbf Camera, supplied by MBF Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/builder+for+java+mbfj/pmc02832169-150-16-14?v=MBF+Bioscience
Average 90 stars, based on 1 article reviews
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Image Search Results


Diminished efficacy with shorter and later treatment. Five weeks of treatment once daily with 50 mg/kg LM11A-31 (n = 5) beginning at 17 months of age partially prevented and/or reversed the decrease in cholinergic neuron area in the MS and VDB seen in the vehicle treated mice (n = 4). Cholinergic neurons were identified by ChAT immunoreactivity and an unbiased estimate of area was obtained using Microbrightfield (MBF) software. A significant decrease in cell area measurements was seen in all groups (t-test, (n = 8) vs 18 months (n = 4), p values = 0.018–0.001). Inhibition and/or reversal of loss of cell size after five weeks of treatment was partial and reached significance only in the composite data for MS + VDB (t-test, treated vs vehicle, p = 0.032). Values are expressed as mean + sem.

Journal: Scientific Reports

Article Title: Modulation of the p75 neurotrophin receptor suppresses age-related basal forebrain cholinergic neuron degeneration

doi: 10.1038/s41598-019-41654-8

Figure Lengend Snippet: Diminished efficacy with shorter and later treatment. Five weeks of treatment once daily with 50 mg/kg LM11A-31 (n = 5) beginning at 17 months of age partially prevented and/or reversed the decrease in cholinergic neuron area in the MS and VDB seen in the vehicle treated mice (n = 4). Cholinergic neurons were identified by ChAT immunoreactivity and an unbiased estimate of area was obtained using Microbrightfield (MBF) software. A significant decrease in cell area measurements was seen in all groups (t-test, (n = 8) vs 18 months (n = 4), p values = 0.018–0.001). Inhibition and/or reversal of loss of cell size after five weeks of treatment was partial and reached significance only in the composite data for MS + VDB (t-test, treated vs vehicle, p = 0.032). Values are expressed as mean + sem.

Article Snippet: Sections were coded to reduce bias and Microbrightfield (MBF) unbiased stereology was used for the collection of cellular data.

Techniques: Software, Inhibition